Floral visits of the wild bee, Lithurgus atratus, impact yield and seed germinability of okra, Abelmoschus esculentus, in Sri Lanka

Shashini Perera; Inoka Karunaratne

doi:10.26786/1920-7603(2018)20

Authors

Shashini Perera University of Peradeniya
Inoka Karunaratne Department of Zoology Faculty of Science University of Peradeniya Sri Lanka

DOI:

https://doi.org/10.26786/1920-7603(2018)20

Abstract

Bee-pollinated crops in landscapes with a low abundance of bees suffer from insufficient pollination. The present study investigates the effect of wild bee pollination on fruit and seed production in okra, Abelmoschus esculentus (L.) Moench. The study was conducted in a home garden, where an okra field was established for three pollination trials each to include 40 okra plants. For each trial, three sets of 25 flower buds were selected and tagged. One set was covered to deter bees, another set was kept open to enable bee visits and the other set was cross-pollinated by hand. Of the two species of bees, Tetragonula iridipennis (Smith) visited flowers for nectar while Lithurgus atratus Smith collected and carried pollen grains. The period of stigma receptivity and pollen availability coincided with the highest activity of L. atratus from 10.00 a.m. to 12.20 p.m. Bee-pollinated flowers had significantly enhanced okra pod length and diameter, seed number and seed germinability compared to the trial with flowers covered to deter bees. Hand pollinated flowers also produced significantly longer pods and a higher number of seeds with higher germinability. Although there was no significant difference in pod length and diameter and seed number between hand pollination trial and bee-pollinated trial, germinability of bee-pollinated seeds was significantly higher. Present study highlights the importance of the wild bee, L. atratus to enhance pod size, seed number and seed germinability in okra in Kurunegala, Sri Lanka.

Author Biographies

Shashini Perera, University of Peradeniya

Department of Zoology

Inoka Karunaratne, Department of Zoology Faculty of Science University of Peradeniya Sri Lanka

Department of Zoology

References

Al Ghzawi AM, Zaittoun ST, Makadmeh I, al. Tawaha ARM (2003). The impact of wild bee on the pollination of eight Okra genotypes under semi-arid Mediterranean conditions. International Journal of Agriculture and Biology 5 (4):408-410.

Angbanyere MA, Baidoo PK (2014). The Effect of Pollinators and Pollination on Fruit Set and Fruit Yield of Okra (Abelmoschus esculentus (L.) Moench) in the Forest Region of Ghana. American Journal of Experimental Agriculture 4(9): 985-995.

Azo’oEla M, Ali M, Fohouo FT, Messi J (2012) The importance of a single floral visit of Eucara macrognatha and Tetralonia fraternal (Hymenoptera: Apidae) in the pollination and the yields of Abelmoschus esculentus inMaroua, Cameroon. African Journal of Agricultural Research 7(18):2853-2857. DOI: 10.5897/AJAR12.359.

Azo’o Ela M, Tchuenguem Fohouo FN, Messi J (2011) Influence of the foraging entomofauna on okra (Abelmoschus esculentus) seed yields. International Journal of Agriculture Biology 13:761-765.

Blaauw BR, Isaacs R (2014) Flower plantings increase wild bee abundance and the pollination services provided to a pollination-dependent crop. Journal of Applied Ecology 51:890–898.

Cane JH, Sipes S (2006) Characterizing floral specialization by bees: analytical methods and revised lexicon for oligolecty in: Waser NM, Ollerton J (Eds.), Plant-pollinator interactions: from specialization to generalization, The University of Chicago Press, Chicago, pp. 99–122.

Free JB (1993) Insect pollination of crops. London: Academic Press.

Freitas BM, Pereira JOP (2004) Solitary Bees- Conservation, Rearing and Management for Pollination. Imprensa Universitária, Fortaleza, CE, Brazil.

Hamon S, Koechlin J (1991) The reproductive biology of Okra. Self-fertilization kinetics in the cultivated Okra (Abelmoschus esculentus), and consequences for breeding. Euphytica, 53:49-55.

Hamon S, Koechlin J (1991) The reproductive biology of Okra: Self-fertilization kinetics in the cultivated Okra (Abelmoschus esculentus), and consequences for breeding. Euphytica 53:49-55.

Ige OE, Eludire MO (2014) Floral Biology and Pollination Ecology of Okra (Abelmoschus esculentus L. Moench). American International Journal of Biology 2(2):01-09.

Karunaratne WAIP, Edirisinghe JP (2008) Key to the identification of common bees of Sri Lanka. Journal of the National Science Foundation. 36(1):75-97.

Karunaratne WAIP, Edirisinghe JP, Gunatilleke CVS (2005a) Floral relationships of bees in selected areas of Sri Lanka. Ceylon Journal of Science 34:27–45.

Karunaratne WAIP, Edirisinghe JP, Pauly A (2005b) An updated checklist of bees of Sri Lanka with new records. Publication No. 23. MAB Handbook and Occasional paper series, National Science Foundation, Sri Lanka.

Karunaratne WAIP (2004) Taxonomy and natural history of bees in selected areas of Sri Lanka. PhD thesis (unpublished). PGIS, University of Peradeniya, Sri Lanka.

Klein AM, Vaissière BE, Cane JH, Dewenter IS, Cunningham Saul A, Kremen C, Tscharntk T (2007) Importance of pollinators in changing landscapes for world crops. Proceedings of the Royal Society B: Biological Science 274:303–313.

La Duke JC, Dobley J (1995) The chloroplast DNA-based phy-logeny of the Malvaceae. Systematic Botany 20: 259-271.

Liu H, Pemberton RW (2009) Solitary invasive orchid bee outperforms co-occurring native bees to promote fruit set of on invasive Solanum. Oecologia159:515-525.

Lotito S and Quagliotti L (1991) Laboratory tests in relation to emergence of okra (Abelmoschus esculentus L. Moench.) seeds at sub-optimal temperatures. Advances in Horticultural Science 5(4): 149-152

McGregor SE (1976) Common vegetables for seeds and fruits. In: Agric. Handbook No. 496 Insect pollination of cultivated crop plants. U.S. Department of Agriculture. Washington, pp. 538-540.

Michener CD (2007) Bees of the World.2nd Edition, The Johns Hopkins University Press, Baltimore.

Michener CD (1974) The social behavior of the bees. Cambridge (MA): Harvard University Press.

Njoya MT, Wittmann D, Schindler M (2005) Effect of Bee Pollination on Seed Set and Nutrition on Okra (Abelmoschus esculentus) in Cameroon. Proceedings of the International Ollerton J, editors. Plant-pollinator interactions: from specialization to generalization. Chicago (IL): The University of Chicago Press, pp 99–121.

O'Toole C, Raw A (1991) Bees of the World. Blandford Publishing, London, UK.

Potts SG, Vulliamy B, Roberts S, O’Toole C, Dafni A, Ne’EmanG, Willmer P (2005) Role of nesting resources in organizing diverse bee communities in a Mediterranean landscape. Ecological Entomology 30:78–85.

Robertson C (1925) Heterotropic bees.Ecology 6:412–436.

Roubik DW (1989) Ecology and natural history of tropical bees. New York: Cambridge University Press.

Rust RW, Cambon G, Grossa JT, Vaissiere BE (2004) Nesting Biology and Foraging Ecology of the Wood-boring Bee Lithurgus chrysurus (Hymenoptera: Megachilidae) Journal of the Kansas Entomological Society 77(3): 269-279

Sampson BJ, Pounders CT, Werle CT, Mallette TR, Larsen D, Chatelain L, Lee KC (2016) Aggression between floral specialist bees enhances pollination of Hibiscus (section Trionum: Malvaceae). Journal of Pollination Ecology 18(2):7-12.

Silva TSHE, Diyes GCP, Karunaratne WA IP, Edirisinghe JP (2018) Rediscovery of Tetragonula praeterita after 1860: an unremarked common stingless bee endemic to Sri Lanka. Journal of the National Science Foundation of Sri Lanka 46 (1):109 – 113, DOI: 10.4038/jnsfsr.v46i1.8271

Strickler K (1979) Specialization and foraging efficiencies of solitary bees.Ecology 60:998-1009.

Vaissière BE, Vinson SB (1994) Pollen morphology and its effect on pollen collection by honey bees, Apis mellifera L. (Hymenoptera: Apidae), with special reference to upland cotton, Gossypium hirsutum L. (Malvaceae), Grana 33(3): 128-138, DOI:10.1080/00173139409428989

Vitale N, Vazquez DP (2017) Ecology and nesting biology of the wood-boring bee Trichothurgus laticeps (Hymenoptera: Megachilidae) in a Monte desert reserve in mid-western Argentina. Apidologie 48:31–40, DOI: 10.1007/s13592-016-0446-6

Wcislo WT, Cane JH (1996) Floral resources utilization by solitary bees (Hymenoptera: Apoidea) and exploitation of their stored food by natural enemies. Annual Reviews of Entomology 41: 257-286.

Winfree R, Williams NM, Dushoff J, Kremen C (2014) Species abundance, not diet breadth, drives the persistence of the most linked pollinators as plant-pollinator networks disassemble. American Naturalist 185:600-611.

Wodehouse RP (1935) Pollen Grains: their structure, identification and significance in Science and Medicine. New York. McGraw Hill. USA.