TY - JOUR AU - Thomson, James D. AU - Ogilvie, Jane E. AU - Makino, Takashi T. AU - Arisz, Angela AU - Raju, Sneha AU - Rojas-Luengas, Vanessa AU - Tan, Marcus PY - 2012/10/31 Y2 - 2024/03/28 TI - Estimating pollination success with novel artificial flowers: Effects of nectar concentration JF - Journal of Pollination Ecology JA - J Poll Ecol VL - 9 IS - 0 SE - Notes on Methodology DO - 10.26786/1920-7603(2012)14 UR - https://pollinationecology.org/index.php/jpe/article/view/185 SP - 108-114 AB - We developed novel artificial flowers that dispense and receive powdered food dyes as pollen analogues while their nectar is replenished by capillary action. Dye receipt, which can be measured colourimetrically, is a direct surrogate for pollen receipt or female reproductive success, but can also serve to compare pollen donation (male reproductive success) from flowers with different colours of dye. By allowing captive bumble bee colonies to visit large arrays of such flowers, we investigated whether total dye receipt depended on the sugar concentration of a flower’s nectar. Estimating pollen transfer, rather than simply visitation rate, is appropriate for this question because flowers with more concentrated nectar might accrue more pollen not only through higher visitation rates but also through longer visits that transfer more pollen per visit. Flowers with richer nectar did receive more dye regardless of their spatial arrangement, but the effect was greatest when rich and poor flowers were segregated in large blocks, as opposed to being intermingled. ER -